Determination of Alpha-Protein, Carcino-embryonic antigen and c-reactive protein in patients with malignant liver pathology

• Author: Petrit Gecaj
• Position: Biochemist
• Pages: 98-103

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5

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3

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ovem

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er

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2019

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cademic Journal o

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Business,

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dministration, Law and Social Science

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II

PCCL Publishin

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, Graz-

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ustri

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SSN 2410-391

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cces online at www.ii

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ccl.or

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Determination of Alpha-Protein, Carcino-embr

y

onic antigen and c-reactive

protein in patients wit

h

ma

l

ignant

l

iver pat

h

o

l

og

y

Petrit Geca

j

B

io

ch

e

m

ist

Ab

str

a

ct

I

n this paper there are presented values of

A

FP, C

EA

, CRP in malignant liver pathologies.

A

lpha-Fetoprotein

A

FP is an oncofetal protein. The pathological increase in

A

FP is increased

i

n malignant liver pathologies, testicular cancer and ovarian cancer.

A

signi

f‌i

cant increase in

A

FP is noted in hepatocellular carcinoma, with AFP up to 3000 ng / ml. AFP is a useful tumor

m

arker in the diagnosis and monitoring of hepatocellular carcinoma treatment.

A

FP levels in

b

lood sera above 3000 ng / ml a est to the presence of hepatocellular carcinoma. In laboratory

practice

A

FP is measured by analysis of immunoassay techniques.

I

n 20 patients diagnosed

with liver cancer,

A

FP was measured in blood serum using the

E

L

I

S

A

analytical method.

M

easurements were per

f

ormed on the

E

L

I

S

A

Human R

EA

D

E

R HS micro

f

otometer.

Study data indicate that in malignant liver pathologies, there is a statistically signif‌i cant

i

ncrease in the concentration of

A

FP in blood serum.

(

P <0.001

).

C

arcinoembryonic antigen (C

EA

) is a glycoprotein with molecular weight 180 KD. This

g

lycoprotein is synthesized during normal

f

etal development in the gastrointestinal and

pancreas tracts.

I

t is then secreted into the circulatory system. C

EA

is widely used as a marker

o

f gastrointestinal cancer.

O

ther malignant pathologies can cause high levels of C

EA

.

A

mong

t

hese malignant pathologies are liver cancer. C

EA

concentration was measured in the above

20 patients, in whom

A

FP was also determined. Determination of C

EA

was performed by

i

mmuno

f‌l

uorescence method on nitro cellulose supernatant. Measurements were made with

m

icrof‌l uorometers equipped with parametric microchips.

I

t results that in malignant liver

pathologies the concentration of C

EA

in blood serum increases statistically signif‌i cantly (P

<

0.001

)

.

C

-Reactive Protein (CRP) is a protein that is synthesized in the liver during the acute phase

of

acute processes.

I

n many cases, malignant pathologies are associated with in

f‌l

ammatory

phenomena.

I

n cases where malignant pathology is associated with inf‌l ammatory processes,

t

h

e

d

e

t

e

rm

i

n

a

t

io

n

o

f CRP

i

n bl

oo

d s

e

r

u

m

i

s

u

s

e

f

u

l. T

o

m

ea

s

u

r

e

CRP c

o

nc

e

ntr

a

t

io

n

i

n bl

oo

d

serum, in 20 patients with malignant liver pathology we selected the immunoturbidimetric

m

ethod. Results show that in malignant liver pathologies the concentration of CRP in blood

serum is also signi

f‌i

cantly (P <0.001) increased, which proves the presence o

f

necrosis and

i

nf‌l ammation in these pathologies

.

K

e

y

words:

A

lpha-Protein, Carcino-embryonic antigen, C-reactive protein, malignant liver

pathology

.

I

.

V

alues of Alpha-Fetoprotein in Malignant Liver Patholog

y

A

l

p

ha-Feto

p

rotein

A

FP is an onco

f

etal

p

rotein. De

p

ending on the anal

y

tical techni

q

ue

used to measure AFPS levels, its normal blood values are less than 10 ng / ml. A

p

h

y

siological increase in

A

FP is observed in gravidit

y

. Most

p

regnant women have

a high concentration o

f

al

p

ha-

f

eto

p

rotein in their blood serum. The

p

athological

increase in

A

FP is increase

d

in ma

l

ignant

l

iver

p

at

h

o

l

ogies, testicu

l

ar cancer an

d