Commission Directive 92/95/EEC of 9 November 1992 amending the Annex to the Seventh Directive 76/372/EEC establishing Community methods of analysis for the official control of feedingstuffs

• Published date: 13 November 1992
• Subject Matter: Approximation of laws,Veterinary legislation
• Official Gazette Publication: Official Journal of the European Communities, L 327, 13 November 1992

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31992L0095

Commission Directive 92/95/EEC of 9 November 1992 amending the Annex to the Seventh Directive 76/372/EEC establishing Community methods of analysis for the official control of feedingstuffs

Official Journal L 327 , 13/11/1992 P. 0054 - 0062
Finnish special edition: Chapter 3 Volume 45 P. 0182
Swedish special edition: Chapter 3 Volume 45 P. 0182

COMMISSION DIRECTIVE 92/95/EEC of 9 November 1992 amending the Annex to the Seventh Directive 76/372//EEC establishing Community methods of analysis for the official control of feedingstuffs

THE COMMISSION OF THE EUROPEAN COMMUNITIES,

Having regard to the Treaty establishing the European Economic Community,

Having regard to Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feedingstuffs (1), as last amended by the Acts of Accession of Spain and Portugal, and in particular Article 2 thereof,

Whereas the Seventh Commission Directive 76/372/EEC of 1 March 1976 establishing Community methods of analysis for the official control of feedingstuffs (2), as amended by Directive 81/680/EEC (3), prescribes the methods to be used for the determination of aflatoxin B1;

Whereas there are grounds for adapting these methods in the light of advances in scientific and technical knowledge; whereas it is advisable particularly to have available a method of controlling the very low limits for aflatoxin fixed by Council Directive 74/63/EEC of 17 December 1973 on the fixing of maximum permitted levels for undesirable substances and products in animal nutrition (4), as last amended by Directive 91/132/EEC (5);

Whereas it is also advisable to have available a method of determining aflatoxin B1 in the presence of interfering substances, such as citrus pulp;

Whereas the measures provided for in this Directive are in accordance with the opinion of the Standing Committee for Feedingstuffs,

HAS ADOPTED THIS DIRECTIVE:

Article 1

The Annex to Directive 76/372/EEC is amended in accordance with the Annex to this Directive.

Article 2

Member States shall bring into force not later than 1 October 1993 the laws, regulations and administrative provisions necessary to comply with the provisions of this Directive. They shall fortwith inform the Commission thereof.

When Member States adopt these provisions, they shall contain a reference to this Directive or shall be accompanied by such reference at the time of their official publication. The procedure for such reference shall be adopted by the Member States.

Article 3

This Directive is addressed to the Member States. Done at Brussels, 9 November 1992. For the Commission

Ray MAC SHARRY

Member of the Commission

(1) OJ No L 170, 3. 8. 1970, p. 2. (2) OJ No L 102, 15. 4. 1976, p. 8. (3) OJ No L 246, 29. 8. 1981, p. 32. (4) OJ No L 38, 11. 2. 1974, p. 31. (5) OJ No L 66, 13. 3. 1991, p. 16.

ANNEX

I. Under Part A 'One-dimensional thin layer chromatographic method' the text at point 1 'Purpose and scope' is replaced by the following text:

'1. Purpose and scope

The method makes it possible to determine the level of aflatoxin B1 in raw materials and straight feedingstuffs. This method can not be applied in the presence of citrus pulp. The lower limit of determination is 0,01 mg/kg (10 ppb).

In the presence of interfering substances it is necessary to repeat the analysis using method B (high performance liquid chromatography).'

II. Under Part B 'Two-dimensional thin layer chromatographic method' the text is replaced by the following:

'B. DETERMINATION OF AFLATOXIN B1. HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD

1. Purpose and scope This method is for the determination of aflatoxin B1 in animal feedingstuffs including those containing citrus pulp. The lower limit of determination is 0,001 mg/kg (1 ppb). 2. Principle The sample is extracted with chloroform. The extract is filtered and an aliquot portion is purified on a Florisil cartridge followed by a C18 cartridge. The final separation and determination is achieved by high performance liquid chromatography (HPLC) using a reversed phase C18 column, followed by post-column derivatization with iodine in water, and fluorescence detection. Note: mycotoxins are extremely toxic sustances. Manipulations should be performed in a designated fume cupboard. Special precautions should be taken when toxins are in a dry form because of their electrostatic nature and resulting tendency to disperse in working areas. 3. Reagents 3.1. Chloroform, stabilized with 0,5 to 1,0 % of ethanol, by mass. See observation 10.2. 3.2. Methanol, HPLC grade for preatation of 3.6. 3.3. Acetone. 3.4. Acetonitril, HPLC grade. 3.5. Eluting solvents: Prepare one day before use, or remove air in the solvents ultrasonically. 3.5.1. Mixture of acetone (3.3) and water, 98+2 (v+v). 3.5.2. Mixture of water and methanol (3.2), 80+20 (v+v). 3.5.3. Mixture of water and acetone (3.3), 85+15 (v+v). 3.6. Mobile phase for HPLC Mixture of water, methanol (3.2) and acetonitril (3.4), 130+70+40 (v+v+v). NB The composition of the mobile phase solvent may need to be...